I remember a paper saying that the hok/sok system of e.coli also works in yeast too. I assume, if it is toxic to bacteria and yeast, it may be aldo toxic to mammalian cells...
should have a look on pKlac, I guess that's exactly what you'd want...
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Re: [DIYbio] DIY and open Chymosin (Cheese Hacking) - anyone has some more experience
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