Re: [DIYbio] DIY media for bioluminescente algae

> Probably because he can't then legally grow them outdoors in a pond
> where it'd look cool.

Exactly... 


> I wonder are there freshwater relatives of those bugs in the wild
> though, and whether certain salt concentrations make them receptive to
> DNA.

Haha, that would be fine... 

But they're eukaryotes, so only (legal) means to achieve this would be cross-breeding with freshwater algae? There are at least four genes involved, one that produces the light organelles (scintillons), luciferase, luciferin (or a few genes), a luciferin binding protein. 
Perhaps also a circadian rythm gene which activates the glow. And something that drops the pH in the scintillons, (IIRC, they need pH 6)

>Yet more of the illogic surrounding current GMO regulations in Europe,
which assumes that outside of human intervention, genetic modification
never occurs.

You say it... Crazy, gotta move to US^^


On Thu, Aug 1, 2013 at 12:36 PM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
Probably because he can't then legally grow them outdoors in a pond
where it'd look cool.

I wonder are there freshwater relatives of those bugs in the wild
though, and whether certain salt concentrations make them receptive to
DNA. Is it really your fault if some naturally competent bacteria were
to encounter some dead cousins under the right natural conditions? :P
(Don't do this)

Yet more of the illogic surrounding current GMO regulations in Europe,
which assumes that outside of human intervention, genetic modification
never occurs.

On Thu, 1 Aug 2013 02:23:44 -0700 (PDT)
Ravasz <ravaszmeister@gmail.com> wrote:

> How about cloning glow genes into some freshwater algae? :P My guess
> is you'd do better in the long run.
>
> On Wednesday, 31 July 2013 11:20:10 UTC+2, DrBrian wrote:
> >
> > Seawater + garden plant fertilizer?
> >
> > Mega why do you want to grow them in less salty water. Can get
> > contamination with potentially bad bacterias.
> > On 27 Aug 2012 12:31, "loïc lauréote" <loic.l...@gmail.com
> > <javascript:>> wrote:
> >
> >> Hi everybody,
> >> Does anybody knows a DIY liquid media for growing bioluminescence
> >> algae like  *Pyrocystis lunula.
> >> *I grow this algae with a standard laboratory media*, but *it's
> >> too expensive for massive growing*.
> >> *Thank you for yours answer*s.
> >> .
> >> *Thanks
> >>
> >> Best regards*
> >> *
> >>
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