Re: [DIYbio] Re: DIY media for bioluminescente algae

Would be nice to grow them in a freshwater pond of a friend. If not possible, we have to put salt in that pond :D

As they are non-toxic, I guess they would be harmless, yet very cool when your pond glows at night ;)



On Thu, Aug 1, 2013 at 10:49 AM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
On Wednesday, July 31, 2013 1:54:43 AM UTC-7, Mega [Andreas Sturm] wrote:
Does anyone know whether you can adapt the dinoflagellates (P. Fusiformis) to lower salt concentration / freshwater? 

I doubt anyone has tried yet. They do grow very slowly as is though, so they'll take a longtime to evolve to adapt to the lower salt concentration. And even if you can grow them in lower salt, they may grow sooo slow it might not be worth your time.

I'd love to be able to find some way to grow them at much higher concentrations though - that might be feasible. I suspect they're producing some sort of byproduct that limits their own growth. We might be able to figure out what that limiting factor is - or at least narrow down the options - by centrifuging down and fractionating the supernatant.

Another option might be to test a range of marine bacteria in a co-culture with the dinoflagellates, in case one of them can metabolize whatever the dinos are excreting, and allow them to reach higher concentrations. Plenty of known examples of symbiotic relationships between bacteria and dinoflagellates or other algae...

Patrik

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