Re: [DIYbio] Re: restriction digest confirmation

On Wed, Aug 7, 2013 at 2:59 PM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Indeed; with flat-bed gels in particular, if there isn't enough buffer
> atop the gel, the voltage tends to short-circuit through the liquid
> buffer and disproportionately heat the top millimeter of the gel,
> sometimes melting and shifting it along with any DNA contained within.

I hadn't thought of that, I was referring to the edge of the gel being
cooler than the center, so you get smearing in-band unless you use a
capillary where heat transfer is faster thus evening the temperature
difference in the gel greatly. This is one reason why they use 50
micron capillaries for automated Sanger sequencing.

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