RE: Fwd: [DIYbio] Thermal Cycler Based Bacterial Trasformation

Given the average used PCR machine has a ramp time of about 2 degrees per second, it would be a very significant variable, no? The thin walled pcr tubes are made to transfer heat as quickly as possible. A swing from 4c to 42c at 2c/sec would still be 19 seconds. I've managed to get colonies using traditional heat shock with only 10sec exposure. Also has anyone ever proven experimentally that a membrane pore formation is what is actually occurring? Like freeze fracture electron microscopy or something to depict the pores? Just curious.

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