[DIYbio] Re: qPCR fluorescence detection dynamic range

Hello all, first time post.  Thought I would bring a couple of projects to your attention. 

First is out of UC Berkley 

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0070266 

The cool part is they provide in a zip file all of the schematics and source code etc.  This may help answer some of the questions being thrown around here.  

The second is a project I am involved in, we have built a low cost qPCR machine. 

www.bio-meme.com

Josh cant wait to see yours in action. 

Jesse 

On Monday, May 13, 2013 4:04:47 PM UTC-4, Josh W. Perfetto wrote:
Hello,

Does anyone have a sense of what real time PCR instruments typically offer for the dynamic range and resolution of the fluorescence detection (and NOT the dynamic range of the overall detection)? I am trying to determine the minimal requirements for a cheaper machine.

-Josh

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