Hi Patrick,
Is there anyway conduct a perfusion decellurization at home?
I'm attempting to reduce some shop bought organs to a structural scaffold for a project yet have no lab space or equipment.
I am not looking to achieve a particularly high level of precision, but would love any suggestions you may have.
Are there any household products containing trypsin?
On Sunday, 3 November 2013 07:38:20 UTC, Patrik D'haeseleer wrote:
-- Is there anyway conduct a perfusion decellurization at home?
I'm attempting to reduce some shop bought organs to a structural scaffold for a project yet have no lab space or equipment.
I am not looking to achieve a particularly high level of precision, but would love any suggestions you may have.
Are there any household products containing trypsin?
On Sunday, 3 November 2013 07:38:20 UTC, Patrik D'haeseleer wrote:
Great job on the video, Matt! Love the music.
For those of you who haven't been following the Ghost Heart saga: we decellularized a pig heart as a Halloween project, stripping out all the cells by perfusing the heart muscle with trypsin, detergent, bile acid, and oxyclean/ethanol - and lots of water and high saline rounds in between.
The purpose of decellularization an organ is to then reseed the remaining scaffold of connective tissue with a patient's own stem cells, regenerating an intact organ that can then be implanted into the patient without any tissue rejection issues. (see Tissue engineering: How to build a heart). The last part - reseeding with stem cells to regenerate an organ ready for transplantation - is still years of research away from clinical use. We just wanted to have a Ghost Heart in a jar, as a show piece!
You can find the official protocol here:
And here's a wonderful video on Jove showing the procedure:
We took some shortcuts along the way, but for a show piece it came out wonderful. We'll be writing up exactly what we did soon. Meanwhile, check out a selection of the best pics in our Meetup gallery:
http://www.meetup.com/Counter-Culture-Labs/photos/18280182/
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