RE: [DIYbio] Re: DIY Microfluidics Device Manufacturing

I wanted to mess around with the old shrinky dink-pdms route. End goal
is just a learning experience and potentially something to test
protoplast turgor in real time. Not trying to do anything fancy, thanks
a ton for the huge post and effort, Nathan! It would also be
interesting to stream line small volume plant hormone mixes via
gradient diffusion and use the resulting batch of gradient samples in
protoplast growth and regeneration experiments. Time to dig up ye olde
shrinky dink kit. :P Shooting for semi-micro fluidics on the order of
tens to hundreds of uL and cells roughly 50um in diameter.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Nathan McCorkle
Sent: 11/24/2013 10:47 PM
To: diybio
Subject: Re: [DIYbio] Re: DIY Microfluidics Device Manufacturing
http://envisiontec.com/products/3d-bioplotter/

MakerBot says 2.5 micron Z and 11 micron XY resolution, but I know
most people use 100 micron setting for the Z, so I'm not sure if
anyone has used it at the limit. A quick google doesn't anything much
for 'microfluidics makerbot' (without the quotes).

These caught my eye, but only lead me to the previously posted links.

http://www.reddit.com/r/3Dprinting/comments/1do7pm/recommendations_for_a_high_resolution_3d_printer/
http://www.practicalmachinist.com/vb/general/3d-printing-makerbot-replicator-2-way-could-actually-useful-252817/index3.html

On Sun, Nov 24, 2013 at 7:35 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Sun, Nov 24, 2013 at 6:31 PM, BioBot <cchau2010@gmail.com> wrote:
>> Maybe easiler just to print it using 3D printer and use it as a master...
>> Robo
>
> Hmm, you certainly wouldn't be able to get channels closely spaced
> together, based on the diameter extruded. Section 3.5 is on FDM (fused
> deposition modelling):
> http://diyhpl.us/~nmz787/pdf/A_review_on_3D_micro-additive_manufacturing_technologies.pdf
>
> Also, Dr. Jordan Miller would probably be the most experienced here on
> the list. I'll cc him.
>
>>
>>
>> On Friday, November 22, 2013 10:08:43 PM UTC-5, Dieter wrote:
>>>
>>> Not all fluidics are created equally. I think you'd have a hard time
>>> creating a chemostat with a milled fluidic system. What are you trying to
>>> do?
>>>
>>> On Thursday, November 21, 2013 3:02:54 PM UTC-8, Sebastian wrote:
>>>>
>>>>
>>>> You guys and gals know of anyone working on micro-cnc designs or
>>>> Microfluidics channel layout making protocols. Something like resin casting,
>>>> cnc milling, etc. I had a project on the backburner and wanted to give some
>>>> chemostat-esque apparatuses (aparati?) a shot. Just got a hankering for
>>>> making tiny channels in plastic and playing with chemostats. Thanks!
>>>> Sent from Windows Mail
>>>>
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>
>
> --
> -Nathan



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-Nathan

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