You could also optomize the RBSs of all the pVIB proteins, https://salis.psu.edu/software/. The RBS will promote over expression of the pVIB proteins, even though that might screw over your cells because of such high expression
And also get better promoters, you can PCR them from the genome... all you gotta do is boil a tube of E coli and use it for PCR xD
On Tuesday, December 3, 2013 6:44:13 AM UTC-8, Mega [Andreas Stuermer] wrote:
-- And also get better promoters, you can PCR them from the genome... all you gotta do is boil a tube of E coli and use it for PCR xD
On Tuesday, December 3, 2013 6:44:13 AM UTC-8, Mega [Andreas Stuermer] wrote:
I didn't add glycerol to it, and also got glowing from sea fish.
Btw., you could add myristic acid which may enhance the glow.
Which brings me toa new idea, supplementing glowing E. Coli with the fatty acid, to get pVIB on steroids. :D
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