On 2 December 2013 01:58, V Shenoy <vshenoy12@gmail.com> wrote:
Hi I am new to Diy bio, and I was wondering if I could get some help from you guys. I was wondering if any of you had any advice on trying to grow specific types of bacteria. By this I mean that I want to grow bacteria with specific traits. I am trying to do this on a fairly low budget (so no buying bacteria) but I have sufficient lab space, a microscope, and other equipment needed for growing bacteria. I would really appreciate it if you could help me. Thanks!
Hi,
I am probably not that far ahead, because I only got a microscope a few months ago, but I found that once I could make up agar plates and inoculate with samples confidently, I was able to take swabs off all sorts of stuff and inspect it under the microscope.
I am probably not that far ahead, because I only got a microscope a few months ago, but I found that once I could make up agar plates and inoculate with samples confidently, I was able to take swabs off all sorts of stuff and inspect it under the microscope.
Once I had worked out how to get the bacteria to spread out nice and evenly on the agar, it was possible to identify them as cocci, rods, staphylocci, dicocci, etc just under the 40x objective/20x ocular magnification.
After that I started to learn about gram staining, and gram positive and negative, and now I am looking at using growth medium and ampicillin to select for particular strains.
However if I as starting again, this time I'd get the basic handling of bacteria in a sterile fashion as basic skill first, and then move on to selecting for traits when you are confident.
Cheers,
Tom
Cheers,
Tom
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