[DIYbio] expressing your own DNA, autoimmune question

We've talked before about GFP tattoos, insufflation of DNA showing up
in the liver and lymph nodes (1) and all the talk about different
phage vectors.

None of the questions have talked much about self-provided DNA (clonal).

In the case of Cystic Fibrosis, the ion channel gene is messed up.
Does changing a few basepairs to fix the gene, then re-introducing
that gene in some kind of degradable vector set off the non-self
immune system?

By degradable vector I mean it doesn't replicate or integrate, maybe
this is just naked DNA with some synthetic mods to resist immediate
degradation, or naked DNA with some protein packaging.

Seems like a daily inhaler-full of linear/plasmid DNA would be better
than percussion:
https://en.wikipedia.org/wiki/Cystic_fibrosis#Other_treatments_for_lung_disease
https://en.wikipedia.org/wiki/Percussion_(medicine)

Now assuming an inhaled DNA takes the same route as the insufflated,
rather than affecting the lungs, we move on to other genes. Maybe
vitamin-C genes, GULO is 19% different compared to a functional rat
gene (2), not sure how many bp would need to be tweaked to get human
form to function.

Now I don't know whether the GULO pseudogene is even currently
expressed, if not, then it seems that a semi-clonal functional copy
would show up as non-self. Seems that if you had some constitutively
*repressed* gene, the same logic would apply.

So my last question is, what if you simply want to over-express genes
already expressed in the body? Maybe they don't normally express in
the liver/lymph, or maybe their are multiple genes that normally
express in different organs or vesicles.

It would probably be bad (or maybe benign) to express a clonal lung
ion-transporter in your liver (I don't have cystic fibrosis, so I
assume it would show up as self), but would it lead to an autoimmune
situation?

1.
http://diyhpl.us/~bryan/papers2/bio/Nasal%20absorption%20and%20biodistribution%20of%20plasmid%20DNA.pdf

2.
http://www.ece.drexel.edu/gailr/group/drexel%20summer%20mentorship%20poster.ppt.pdf

--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9Jcu9wi40_N%2Bvv1vkRaOc15X9d7Ln_CBrJ328VVBaVKUw%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.