The sacB promoter, which is activated by sucrose, would express lets say, cro. The cro would then repress the mazF gene. Once you cut the bacteria's sugar rations, cro no longer gets expressed, so mazF could be expressed
-- The sacB promoter would also control comK, which would make it so you make comp cells in the presence of sucrose. Double whammy!
On Thursday, January 2, 2014 1:43:32 PM UTC-8, Mega [Andreas Stuermer] wrote:
On Thursday, January 2, 2014 1:43:32 PM UTC-8, Mega [Andreas Stuermer] wrote:
Sounds awesome! But how do you surpress the protein feom killing the cell befire transformation? You gotta amplify it first carrying the lethal gene.Maybe incubating them with some inhibitor?
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