I don't think it could replace your gut flora. You have strains of e coli in there which produce a lot of other toxins and would quikly kill them off. Maybe even such which carry a natural copy of colicinV?
Btw, would it do harm if the e coli in your gut were k12 derivates with GFP? Rather not I gzess. And colicin eon't harm the lactobacilli either?
Regarding antibiotic markers: Amp may be safer. that's the one side of the medal. On the ozher hand, the European Union drasicalliestlly restricts GMO. Self-cloning organism (cisgene) are not regulated. so you either do this or nothing at all^^
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[DIYbio] Re: [diybio-eu] Re: IndieBB Crowdfunding Campaign: Help me make a great beginner's kit for DIYbio/synbio!
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