Re: [DIYbio] Re: Genetic engineering at home

Mike, could you instead of transfecting the thymus/spleen, try instead
for bone marrow? I know bone marrow transplant is effective at curing
HIV, but also that it's quite risky surgery. Maybe a transfection
would be like laproscopy, where they only make a small incision to do
surgery, limiting risk and damage?

Also, even though this stuff isn't DIY-level due to safety concerns,
there are lots of young people here so I do think this discussion is
healthy. Lot's of DIY folk work or sometimes communicate with
academia, so there is plenty of interdisciplinary idea formation
potential here.

On Fri, Jan 3, 2014 at 12:12 PM, Mike Horwath <mikeh169@gmail.com> wrote:
> Saw Mega's post after I made my post. Basically, it's too late for central
> tolerance because you already have T-cells likely to react with
> GFP...transfecting thymus/spleen won't help. You can try to create
> peripheral tolerance, say by injecting immunosuppressive cytokines, but even
> the professionals don't have a good handle on it. You're pretty much
> playing russian roulette.
>
> Gene therapy DOES have potential applications beyond miRNA. Increase
> expression by adding extra copies of a gene, cut it out entirely with
> CRISPRs, or put in a new variant of a protein that's similar enough to
> existing proteins not to create a response.
>
> However, viral self-engineering is really not where DIYbio should be
> focusing...it's difficult and dangerous, questionably legal, possibly
> unethical. I think DIYers can contribute by taking part in the public
> debate, and helping to develop tools for self-diagnosing whether gene
> therapy could help a person. But if you actually want to do gene therapy
> with viruses...think about entering academia or biotech!
>
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--
-Nathan

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