Invitrogen value oligos ftw
On Friday, January 31, 2014 8:37:58 AM UTC-8, Sebastian wrote:
-- I think its true that snapgene is trying to be the jack of all trades... I wish there was opensource software (hint hint) just like theirs so you could program your own plugins. So far all the free ones i have tried don't even marginally compare to it. On the topic of primers, I wish they added more support in for primers... last time I asked about it they gave this, which was pretty good explanation
-Koeng
On Friday, January 31, 2014 8:37:58 AM UTC-8, Sebastian wrote:
I have the licensed version for my company but I really don't trust the primer Tm calculation since it does not use the "latest" calculation methods. If you take your primer made via snapgene and plug it into a more sophisticated program like Primer 3 Plus, which checks for hairpins, self annealing, and a ton of other user editable parameters, the Tm will vary from program to program. I've had issues designing primers via snapgene's minimalistic calculation approach. A few of my longer primers ~40bp failed. Later analysis with Primer 3 Plus showed a high chance of hairpin. When I redesigned it using only primer 3 it worked like a charm. I absolutely LOVE SnapGene but its trying to be jack of all trades. Even though I get my primers for a flat $5 a pop, its still a waste to have them fail due to silly avoidable design flaws...partly my own fault :P
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Koeng
Sent: 1/31/2014 11:27 AM
To: diy...@googlegroups.com
Subject: [DIYbio] Re: Primer Software?I use snapgenes built in software. Just outline dna and it gives TM. I highly recommend the trail version--
On Friday, January 31, 2014 5:24:52 AM UTC-8, Sebastian wrote:I use Primer 3 Plus. Its nice and simple. What are your favorites? Anyone ever try premier biosoft's program? Did anyone ever get Tm that's way off predicted? Just trying to get an idea of what everyone uses to see if im not missing out on some open source goodness. Thanks!
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
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