Re: [DIYbio] dna purification (once more)

On Wed, Feb 26, 2014 at 8:43 PM, Josiah Zayner <josiah.zayner@gmail.com> wrote:
>
> Are you sample limited?
> If not why not try and grow up one culture for genomic DNA purification and
> another for plasmid purification.
>

Not really sample limited. I have a few grams of pelleted cells. These
can't be easily grown, they need some special media. It's a WT
bacteria, probably isolated from a sample.

> Are you sure that band is a plasmid? Have you tried retransforming it and.or
> cleaving it with restriction enzymes or PCR?
>
I am going to guess that it is not a common bacteria. I was not told
anything more about its sequence or features. It needs to be
sequenced, so maybe it is novel? I will have to find out more though.
If it is not plasmid, what else could it be?

> Do you have a spectrophotometer.nanodrop to check the concentration of the
> DNA that was purified?
>
Yes. Miniprep results gave me 100ng/uL. gDNA results show around 20ng/uL.


By the way, when a kit says it purifies genomic dna from bacteria, is
it just chromosomal dna or will it also have plasmid in it?

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