Are you sample limited?
If not why not try and grow up one culture for genomic DNA purification and another for plasmid purification.
Are you sure that band is a plasmid? Have you tried retransforming it and.or cleaving it with restriction enzymes or PCR?
Do you have a spectrophotometer.nanodrop to check the concentration of the DNA that was purified?
If you have access to Phenol, Chloroform and Ethanol you can do a Phenol chloroform purification. Lots of protoocols for this online.
On Wednesday, February 26, 2014 3:24:16 PM UTC-8, phillyj wrote:
On Wed, Feb 26, 2014 at 5:42 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
> Did you try the tried and true methods from sambrook? They're quite cheap
> and easy and not size dependent.
>
No, I haven't looked into it yet. I don't have the book, so is there a
place I can see those protocols? Or at least give me names of the
methods so I can google it.
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