Yea, it turns out the BL21 cells I had didn't have the DE3 lysogen, (it is so annoying, took me a while to figure out that was the problem.)
-- I think one of the other people in my lab have it, thanks!
On Friday, March 28, 2014 9:58:06 PM UTC-7, Josiah Zayner wrote:
On Friday, March 28, 2014 9:58:06 PM UTC-7, Josiah Zayner wrote:
If you are looking for bacteria that express T7 polymerase you should probably ask around for BL21 cells most any modern lab should have some.
On Thursday, March 27, 2014 6:47:26 PM UTC-7, Koeng wrote:Does anyone a good (preferably cheap) place to get a bacteria strain with the T7 RNA polymerase? I am of the RNA polymerase gene... Thanks!-Koeng
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