Re: [DIYbio] Re: nanodrop spectrophotometer cheap build

On 03/27/2014 11:25 AM, CodonAUG wrote:
> The nanodrop's arm also dynamically raises and lowers thus changing the path length based upon the samples concentration.

That's not as I understand it for some basic nanodrop models like the ND-1000.
The patent describes two heights
of the parallel optical faces above each other -- one to compress a drop and wet
both surfaces completely, the other height to pull that drop vertically a little and stop
at a fixed distance.

Since the spectrometer functions to "measure" concentration, how do you use concentration info
before you measure?

Or are you talking a bout a higher end model that makes one measurement,
and if concentration is found to be in a weak range, it sets up for the taller
drop stretched shape and takes a second measurement? That would still be about having fixed distances
for calibration -- not what I think of when I hear "dynamically raises and lowers"...

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