Re: [DIYbio] Re: Transgenic CCD resistant honeybee - need help

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Whoops, you already covered this. :)

How big are the LTR/transposases? Would it be cheaper to synthesise them
rather than subcloning?

On 28/04/14 17:54, Andreas Stuermer wrote:
> In the native bee genome there is no transposase for this transposon. Thus,
> it cannot jump out on its own again.
>
> The vecot would contain LTR ---- Resistance casette ---- LTR ----
> transposase
>
> Transposase cuts at the long terminal repeats, and inserts it into the
> genome. So transposase gene is lost after time.
>
>
> On Mon, Apr 28, 2014 at 6:50 PM, Mega [Andreas Stuermer] <
> masterstorm123@gmail.com> wrote:
>
>> My approach won't make CCD extinct. As CCD is a factor of multiple
>> diseases. But I have peptides that fight Nosema (the most hated unicellular
>> parasites that like 90% of hives have) and other antifungal peptides. To
>> kill off viruses is easy, as most bee viruses are RNA viruses. Silencer RNA
>> destroys them.
>>
>> Actually, some viruses are related, so one silencer RNA can destroy 2 or
>> three viruses.
>>
>> Only this two modifications would decrease mortality rates quite
>> drastically, I assume. Gotta test it.
>>
>>
>> http://en.wikipedia.org/wiki/List_of_diseases_of_the_honey_bee
>>
>> I would be ready to start a company selling those bees. But we got to get
>> EPA approval.
>>
>>
>>
>>
>> One problem is also that you try to kill the Varroa destructor mites
>> (which feed on bees) with insecticides which also harms the bees. By just
>> inserting the bees a zeocin resistance or Kanamycin resistance, you could
>> kill the mites effectively, and the bee is protected.
>>
>>
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