Re: [DIYbio] Re: Transgenic CCD resistant honeybee - need help

In the native bee genome there is no transposase for this transposon. Thus, it cannot jump out on its own again. 

The vecot would contain LTR ---- Resistance casette ---- LTR ---- transposase

Transposase cuts at the long terminal repeats, and inserts it into the genome. So transposase gene is lost after time. 

On Mon, Apr 28, 2014 at 6:50 PM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:

My approach won't make CCD extinct. As CCD is a factor of multiple diseases. But I have peptides that fight Nosema (the most hated unicellular parasites that like 90% of hives have) and other antifungal peptides. To kill off viruses is easy, as most bee viruses are RNA viruses. Silencer RNA destroys them. 

Actually, some viruses are related, so one silencer RNA can destroy 2 or three viruses. 

Only this two modifications would decrease mortality rates quite drastically, I assume. Gotta test it. 

http://en.wikipedia.org/wiki/List_of_diseases_of_the_honey_bee

I would be ready to start a company selling those bees. But we got to get EPA approval.

One problem is also that you try to kill the Varroa destructor mites (which feed on bees) with insecticides which also harms the bees. By just inserting the bees a zeocin resistance or Kanamycin resistance, you could kill the mites effectively, and the bee is protected. 

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http://diyspartanbiotech.files.wordpress.com/2014/03/genetic-engineering-and-synbio-for-beginners-v1.pdf