Attached the method of sperm-mediated transfection.
However, in that specific experiment the DNA was inherited episomally.
There is another paper (I'll send you privately, and anyone else who requests) how chromosomal bee integration works. It already has been done with high efficiency. That should be our least problem, given the right vector (it's commercially available, but a bit expensive)
On Sat, May 3, 2014 at 1:39 PM, 'SC' via DIYbio <diybio@googlegroups.com> wrote:
You would mix raw DNA in with bee semen? How would it be incorporated into the genome?--To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/1e55824a-72e8-4656-92ed-779e1230ce61%40googlegroups.com.
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