[DIYbio] automated fluorescent microscope

Hi,

I am interested in building an automated microscope that would be useful for synthetic biology.  Features would include automated tracking and classification of individual live bacteria.  Videos of the time lapse images would be transmitted and assembled in a PC and post processed with machine learning techniques.  I am a computer engineer so the robotics and machine learning are in my domain.  I want to understand the design for a DIY microscope in the sub 1000 range that would have the capability of creating the images.  

From what I have been researching on the web, I was thinking to build a LED Fluorescent microscope.  I am starting from the microscope design at openlabtools.org.  It uses a Rasberry pi and 5MB raspberry pi camera with an infinity optical system.  I want to add a fluorescent stage to that.  There are some DIY articles about LED Fluorescent microscopes but are lacking details though I continue to search.  If someone could point me in the right direction I would appreciate it.  I need information about where to source low cost LED's and design. 

Mike

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