Suggestion: When you try to grow your frozen cells, start them off in a small overnight culture without antibiotic. Then move them to a culture with antibiotic selection. That will give them a head start to produce the antibiotic-resistance proteins.
Panasonic has a nice, small -80 freezer for about 4K.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/0b96f445-10d5-4536-b298-cbd57edd7915%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment