Re: [DIYbio] Need yeast shuttle vector

the NEB protein expression kit has a plasmid like this... pKLAC2

On Mon, Jun 2, 2014 at 1:40 AM, Marc Juul <juul@labitat.dk> wrote:
> I am part of the Counter Culture Labs crew in Oakland, California, and we're
> looking for a yeast shuttle vector, preferably with some or all of the
> following features:
>
> * URA3 gene
> * Ampicillin resistance gene
> * 2-micro ori
> * An E. coli ori like pUC, pBR322 or f1
> * Alpha factor secretion signal
>
> We realized that we can't use addgene.org since their materials transfer
> agreement would put limits on our use and sharing of constructs.
>
> Anyone have an IP and MTA unencumbered shuttle vector we can have?
>
> --
> marc/juul
>
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--
-Nathan

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