Re: [DIYbio] Re: Bioglowtech news

So the picture on the glowing plant project website says they took a 30 second exposure(http://media.tumblr.com/3b9305a1e68ce53b1af5d04a4ca89e67/tumblr_inline_n6f1we67L21rxiq56.jpg). So I really wanted to see what a 30 second exposure was like because normally on cameras the exposure time is a fraction of a second. So I grabbed my camera and took a piece of a plant and put it in a really dark room. I took one exposure at ISO 3600 at 1 second and the picture was totally dark(first picture below)(Glowing plant uses ISO 4000 so I was trying to be close to that) . Then I took one at 30 seconds and you can see stuff, WHOA! 30 seconds is a looooong exposure and basically makes any tiny amount of light seem huge. I literally could not see the flower with my eyes that you see in the second image! I can't even imagine that one could see the plant glow with the naked eye if that is only how bright it is at 30 second exposure. But maybe barely?


Here is a URL with the photos in case they don't load in the email: https://docs.google.com/document/d/1k9Zag5uwCZO4k46FLewF0q5XUEWShRJSn8w1O4ZPkLk/edit?usp=sharing

They didn't. They are expressing in the nucleus and shipping to the chloroplasts thus circumventing the main technology which is the plastid transformation. Add a YFP to multiply the high energy photons into more, longer wavelength, photons and that makes it different-ish. Still infringing since the initial patent is broad in scope and still borderline criminal since they intend to ship an agrobacterium line, albeit disarmed, which is considered a plant pathogen without a permit per order which is a FEDERAL crime. I Still have not a single clue how all of this is overlooked or disregarded and how the project is aiming for commercial ventures even though they break bioglow's patent in the first claim. BTW, where is the DIY Bio part of all of this? Wasn't this a non-profit attempt to prove it can be done on a "DIY" level?

Either way I can't wait to see the results. Its a bio-drama and will be awesome if somehow a cool plant comes out of it all. Initial sales from bioglow ranged in the $700+ area per plant. Nice profit but really want to hear from the buyers. Was it worth it all? Nicotiana Alta has a relatively short life span and is a bit tricky to grow happy. If I were them I would have made a plastid vector for something more ornamental like petunia. Simple transformations, easy growth, tons of seeds, and various colors.

The one thing I really wish both companies would stop doing is either adding exogenous luciferin or use a 30+ second camera exposure and claim that's the actual glow. Marketing shmarketing. Dont lie to me. If I were them, I'd keep it quiet until I have the real deal and then infiltrate the market all at once. No frills, no empty promises of lighting up the streets, just a solid product or go home.

Some can say you need to sell it to your investors to stay afloat. To that I say, if you make grand claims and your product doesn't sell itself, you've done something wrong. If its really all you say it is, money should flow in faster than banks can keep up. No need to manipulate the truth. Then again, an attitude like mine will never make it in the business world...pardon the digression.

Good luck to both companies. May the glowiest plant win!


Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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From: Koeng
Sent: ‎6/‎2/‎2014 10:17 PM
To: diy...@googlegroups.com
Subject: [DIYbio] Re: Bioglowtech news

I am curious how the glowing plant project got past their patents. Anyone know?

On Monday, June 2, 2014 1:45:35 PM UTC-7, Mega [Andreas Stuermer] wrote:

Hi!

 

Seems the patent holder of glowing plants succeeded in enhancing the bacterial lux operon. Maybe with directed evolution, fluorescent proteins or some other stuff (duplicating lux genes?) .

 

http://www.bioglowtech.com/news.html

The before-after picture looks awesome!!

 

 

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