> Having a non-block with zero mass is even better.
Sure, except that air doesn't transfer heat to your sample nearly as
fast as aluminum. Imagine putting your hand in a 100C oven vs putting
your hand on a 100C frying pan. That's why most thermalcyclers take
into account the volume of the sample and calculate the temperature
instead of just using the temperature of the block.
I'm not saying air won't heat the tubes sufficiently fast. If you are
optimizing for cost then air is the way to go. But heating and
cooling the air rapidly won't necessarily lead to the temperature of
the sample being heated/cooled faster compared to aluminum.
-cory
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Re: [DIYbio] Re: Low cost PCR(under development)
5:45 PM |
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