Re: [DIYbio] Re: Low cost PCR(under development)

On Mon, Jun 30, 2014 at 5:24 PM, John Griessen <john@industromatic.com> wrote:
> Are you meaning measuring temperatures with fine precision inside or next to
> the
> sample vial and comparing heat flow from the heater source by Watts
> delivered?

Not too sure... I feel like the sensor would either be inside the
liquid or maybe non-contact IR? I believe the idea is you command
constant power to the heater (or maybe a constant increase in power),
then watch the temperature, and look for when the temperature curve
isn't smooth. Then do the same thing in reverse cooling the sample.

>
> The usual plastic vial might get in the way -- maybe it should be silicon
> glass,
> and the sample size would have to have more mass than usual, or the machine
> would have to have
> super insulation. Sounds non-low-cost at first glance.

Not too sure.

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