Re: [DIYbio] Re: Low cost PCR(under development)

On Mon, Jun 30, 2014 at 4:05 AM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Remember though, an additional consideration is how homogenous the
> temperature change is through the block, and at what rate the
> temperature change in the block is reflected in the tubes. There would
> be little point doubling the ramp rate of the block if the tubes cooled
> at an only marginally faster rate. Worse, doubling the ramp rate as
> measured by the thermometer may look great on the charts and benchmarks,
> but the block may be unevenly cooling and this can have real
> consequences when you're expecting your samples to experience identical
> conditions.

I'd say that uneven heating or cooling will produce a thermocycler
that will be quite hard to use for normalizing experiments... you'd
never be able to run things in parallel.

The fastest ramp rates I've seen is 15C/sec heating, 12C/sec
cooling... see the pic of an advertisement here:
https://groups.google.com/d/msg/diybio/yttYHgz0gFE/o4Icu_3--lwJ

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