[DIYbio] DIY Protein Separation

Hi! 
Again to my question, there seem to be little guys dealing with FPLC here.

https://groups.google.com/forum/#!topic/diybio/24PZTzije98

Is there a way to do this cheaply at my university, without buying an FPLC machine? 

Thanks god those berberine derivatives are all fluorescent. So detecting will not be a problem.  

What we want to do is: preparing a cell protein extract. Separate those proteins. Add the fractions to berberine, let it incubate,  and do photometry see which turns it into the derivative we want. 

Best,
Andreas

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