[DIYbio] Re: diy electroporation so far?

Put some culture in a cuvette, add vector, add a salt buffer, zap it with an electric field, incubate, see what grows.  I think that's the overview.

Instead of piezo starter, I am eager to have someone look at my HV power supply which can get up to 1800 VDC open circuit.  (A cuvette is an open circuit.)   The diy power supply plans are in the latest issue of BioCoder just out.

You can modify the voltage needed by changing the cuvette size (also known as, the gap creating the electromotive force).  For example many protocols state use of 2.5kV with a 2mm cuvette.  Well, you could use or make a 0.5 mm cuvette (microfludics or standard) and use a lower voltage.  You could flatten a coffee stirrer/drinking straw to a desired width, measured by micrometer.

I guess a piezo starter might work but I would think it would be rather imprecise.  Nature is imprecise too.  But you want to ensure a good result and/or a good yield.


## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
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