Re: [DIYbio] diy electroporation so far?

From what I could tell, the pulse might have been too fast. I think
you'd need a capacitor or inductor, but not sure how much of you'd
need. Small high-voltage caps are cheap, but the ones in all the
academic publications for electroporators were all very large, the
closest I could find from consumer-land was a microwave oven capacitor
(~$10 on ebay). For an inductor, one might be able to get away with a
long piece of wire, but I don't know what thickness and/or how long.

The pulse also could have been oscillating too much, which would add a
diode to the requirements (there are also microwave oven diodes, but
likely that is overkill since the pulse is so short compared to the
1500W a lot of home microwaves use). There's a good amount of
literature on oscillating fields for electroporation, so that's why I
get this feeling.

On Wed, Jul 30, 2014 at 10:49 AM, Jeswin <phillyj101@gmail.com> wrote:
> Hi all,
> What's become of the diy electroporation using the piezo starters? How
> exactly would one go about this? What's the process from beginning to end?
> Would I culture some cells like the NEB competent cells to use for
> electroporation?
>
> I'd like to give it a try if it's not too difficult.
>
> Thanks
>
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--
-Nathan

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