The piezoelectric igniters I sell on my website deliver 18 kilovolts.
I routinely power LEDs with them, so If you need to produce DC, just put an LED in series with the load.
You get a nice flash from the LED each time you click, so you get some feedback that the device is working. Nothing bad happens to the LED -- the current is so low that nothing heats up, and the voltage drops to what the LED can easily handle.
To reduce the voltage, simply put your loads in series. Assuming you want to stick with standard cuvettes, dropping the voltage by a factor of 9 simply means putting eight dummy cuvettes in series with the one that has your critters in it. Or zap 9 cuvettes full of critters at the same time.
You can use cheap off-the-shelf potentiometers as voltage dividers to dial in any voltage you like, from 18 kV down to a hundred volts or so. Again, the current is so low that nothing heats up or gets damaged, so any cheap Radio Shack potentiometer will do fine.
On Wed, Jul 30, 2014 at 11:00 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
From what I could tell, the pulse might have been too fast. I think
you'd need a capacitor or inductor, but not sure how much of you'd
need. Small high-voltage caps are cheap, but the ones in all the
academic publications for electroporators were all very large, the
closest I could find from consumer-land was a microwave oven capacitor
(~$10 on ebay). For an inductor, one might be able to get away with a
long piece of wire, but I don't know what thickness and/or how long.
The pulse also could have been oscillating too much, which would add a
diode to the requirements (there are also microwave oven diodes, but
likely that is overkill since the pulse is so short compared to the
1500W a lot of home microwaves use). There's a good amount of
literature on oscillating fields for electroporation, so that's why I
get this feeling.
--
On Wed, Jul 30, 2014 at 10:49 AM, Jeswin <phillyj101@gmail.com> wrote:
> Hi all,
> What's become of the diy electroporation using the piezo starters? How
> exactly would one go about this? What's the process from beginning to end?
> Would I culture some cells like the NEB competent cells to use for
> electroporation?
>
> I'd like to give it a try if it's not too difficult.
>
> Thanks
>
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