Re: [DIYbio] Nanomicrobiology?

http://scholar.google.com/scholar?q=32.768+khz+crystal+microscopy
http://research.sabanciuniv.edu/10163/1/Scanning_Hall_Probe_Microscopy_(SHPM)_Using_Quartz_Crystal_AFM_Feedback.pdf

crystal is <$1 USD
http://www.newark.com/fox-electronics/nc38lf-327/tuning-fork-32-768khz-12-5pf-thru/dp/61T5432

similar application but some better images of the tips:
http://www.hindawi.com/journals/ijo/2012/591083/
http://diyhpl.us/~nmz787/pdf/Integrating_micro-_and_nanoelectrodes_into_atomic_force_microscopy_cantilevers_using_focused_ion_beam_techniques.pdf

http://diyhpl.us/~nmz787/pdf/Development_of_a_regeneration-type_neural_interface_A_microtube_guide_for_axon_growth_of_neuronal_cells_fabricated_using_focused-ion-beam_chemical_vapor_deposition.pdf

Also this:
http://hansmalab.physics.ucsb.edu/afmhistory.html

On Sun, Jul 27, 2014 at 1:21 PM, Margret Storm <i.am....@gmail.com> wrote:
> This is the paper they linked:
> http://www.uam.es/gruposinv/spmth/papers/2008_Science_332_413_Sugimoto_AFM.pdf
>
> It says: "We performed the AFM experiments (9) in
> dynamic mode under the frequency modulation
> detection scheme (10),  keeping the cantilever
> oscillation amplitude constant. Commercial silicon
> cantilevers, which have very sharp tips at their
> free ends, were used to image the Sn/Si (111)
> […] R30° surface (11) by detecting the
> short-range chemical interaction force between
> the closest tip and surface atoms (9)."
>
> 9: http://arxiv.org/pdf/cond-mat/9706132.pdf
> 10: http://arxiv.org/pdf/cond-mat/0311522v1.pdf
> 11:http://journals.aps.org/pr/abstract/10.1103/PhysRev.141.503 (can't find a
> full paper for this one, sorry)
>
>
> On Sunday, July 27, 2014 3:55:54 PM UTC-4, Nathan McCorkle wrote:
>>
>> Yes I saw that, but it doesn't seem to give a procedure used to produce
>> it, unless that is another paper (maybe referenced in that paper)?
>>
>> On Jul 27, 2014 10:54 AM, "Margret Storm" <i.am....@gmail.com> wrote:
>>>
>>> The third link's second image is of the atom used for manipulation.
>>>
>>> On Sunday, July 27, 2014 2:23:47 AM UTC-4, Nathan McCorkle wrote:
>>>>
>>>> I've been training to operate a Focused Ion Beam milling machine. It can
>>>> mill and deposit metal with a minimum spot size of about 10nanometers. I've
>>>> been making probes with a pipette puller then milling the ends with a
>>>> certain angle at a certain width along the probe tip.
>>>>
>>>> I think FIBs can make AFM probes... I didn't see a picture/diagram of
>>>> the tips they were using in those papers.
>>>>
>>>> On Jul 26, 2014 11:37 AM, "Margret Storm" <i.am....@gmail.com> wrote:
>>>>>
>>>>> These articles are very interesting:
>>>>> http://jb.asm.org/content/184/19/5205
>>>>> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246382/
>>>>>
>>>>> http://www.uam.es/gruposinv/spmth/highlights/2008_Science_322_413/highlight.html
>>>>>
>>>>> Has anyone here experimented with this technology themselves, or do you
>>>>> know anyone who has? What are your thoughts on the applications of the
>>>>> technology where it stands, or on how accessible it is? How viable would it
>>>>> be for modifying DNA/RNA sequences or proteins manually?
>>>>>
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--
-Nathan