Re: [DIYbio] Re: low cost positive pressure laminar flow HEPA filtered box

On 07/29/2014 09:52 AM, Ilya Levantis wrote:
> From this we should start to build up a reasonable data set about likelihood of contamination based on the spatial location in
> the "clean" work area.
>
> I wrote up a blog post about the construction if you're interested: http://biohackspace.org/building-a-diy-flow-hood/

Sounds good. Thanks!

I took a look at your biosafety purpose vertical flow hood and the amount of air intake slots
that pull from the glass shield area seems skimpy.


I think if there really is danger, you would
want a set of ten rows of intake slots in front all creating laminar flow that pulls in some air from the
shield so there is no way bugs get out. What happens as you put your gloved hands through that area?
The laminar flow is all broken up then...

I'd prefer sealed glove boxes that can have a decontamination process done with the gloves
and disinfectants that are always on hand inside, and sterilizing air locks.

But then, I'm not interested in researching pathogens or toxics either.
The most I'd use that design for is to avoid stinky cultures effects on the room
when used with an exhaust duct to the building outside.

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