My last trials with a piezoelectric sparker used two pieces of adhesive-backed aluminum foil on a glass microscope slide, with some wax pencil for a hydrophobic 'corral' between the two foil strips. Seemed like it should work as long as aluminum ions weren't toxic to the cells.
I really think the next step is to add a diode (LED), and a small cap/inductor would be the next step.
On Jul 31, 2014 7:29 AM, "John Griessen" <john@industromatic.com> wrote:
-- On 07/30/2014 05:15 PM, Simon Quellen Field wrote:
You might want to simply place two wires very close to one another inside the cuvette (or Petri dish -- we don't need the cuvette
anymore) and hit the igniter a couple dozen times.
This idea could yield some safer lower powers to electroporate with. The concept is to make the
field strength quantified by the volume between the electrodes and not worry with it being a cuvette.
It could be just small zones of metal separated by a distance.
Then you zap a droplet in any container.
Drawbacks include:
1. needing to reuse such electrodes and so having to clean them.
2. The amount or number of cells affected will vary as the volume you put the electrodes in varies.
3. electrodes are sticking out where they could be dangerous.
So, with the drawbacks considered, I still like the idea of making teeny cuvettes to get the needed currents lower.
They would be used inside an insulated interlocked covered box.
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