Re: [DIYbio] DIY Protein Separation

Thanks!! 

http://www.piercenet.com/product/disposable-plastic-columns

These also seem nice. Easy to use? 

On Sun, Aug 3, 2014 at 8:55 PM, Matt Lawes <matt@insysx.com> wrote:

Chromatography column is the appropriate term. Typically glass or plastic. You may even find prepacked columns of sepharose / sephadex. Go to GE healthcare life sciences and look at sepharose / sephadex technical info to determine which resin (size exclusion) will work best for you.

>matt

Sent from my Verizon Wireless 4G LTE DROID

Andreas Stuermer <masterstorm123@gmail.com> wrote:

Gravity flow sepharose sounds like a way to go. Have you got a keyword how I can find a column where to put in the Sepharose? 

On Sun, Aug 3, 2014 at 8:39 PM, Josiah Zayner <josiah.zayner@gmail.com> wrote:

Gravity flow size exclusion should work fine. As someone else said use Sepharose or Sephadex. You can find it on eBay pretty cheap. If you have money to spare buying old (H)FPLC pumps is pretty cheap on eBay. I think there are people who do (H)FPLC on this list but people probably just don't have time or motivation to run samples for you, including me.

As Cathal said, An ammonium sulfate precipitation is a tried method that has been used for over 60? years. By increasing.decreasing the ammonium sulfate concentration you can precipitate out proteins and try the different fractions.

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