Re: [DIYbio] Re: Relatively safe-to-handle mutagens to expose seeds to?

What is your target for mutagenesis? SNP? Rearrangement in chromosomal architecture?

Chemical mutagen? Radioactive one?

You might want to familiarize yourself with the types of radiations you want to use, if you deem it safe enough.

Although this is rated as not a scientifically reputable site on WOT, some info is better than none. That is not to say that the research may not be viable at all. Some researchers get lured into something like this and have a hard time clearing their name from such sites. This article close to lists what type of radiation gives you the type of results you may want/need for your study.

http://www.hindawi.com/journals/ijpg/2011/314829/

"Compared to radiological methods, chemical mutagens tend to cause single base-pair (bp) changes, or single-nucleotide polymorphisms (SNPs) as they are more commonly referred to, rather than deletions and translocations."

"Among the radiation-based methods, γ-ray and fast neutron bombardment now supersedes X-ray in most applications. Of these, γ-ray bombardment is less destructive causing point mutations and small deletions whereas fast neutron bombardment causes translocations, chromosome losses, and large deletions. "

On Tuesday, August 26, 2014 6:28:30 PM UTC-4, Nathan McCorkle wrote:

On Tue, Aug 26, 2014 at 3:12 PM, Yuriy Fazylov <yuriy...@gmail.com> wrote:
> a real question deserves a real answer
>
> This is how you get mutation on sarcasm level ...
>

I was quick to respond, but we are talking about DNA here... unless
you meant something else by scale. What were you asking? number of
organisms exposed per dose, number of organisms in general, if the
organism was micro/meso/macroscale itself...