Re: [DIYbio] Gas Chromatograph (DIY) maintenance

It can vary.

Can you link the machine you bought?  Or, make model and manufacturer?

Did it come with software?  Is it even working?  Is it the really old kind that just uses a direct print-out?

You'll need a carrier gas and collision gas, so you have to be somewhere where they'll even drop off nitrogen, air, or helium tanks.  

Actually just read it's a GC not a GCMS, so forget collision gasses. We had a GC from the 50's or 60's that still worked.  Pretty simple.  Turn on heat and carrier gas, inject, old school perforated print out, integrate peaks by hand.  

I find LC's to be 100x more useful than GC's, unless you are working with small organic molecules that are volatile. 

Did you have a specific class of metabolites you are wanting to look at with GC?    Where are you going to find controls for your compounds? 

I guess the most important question is what are you even really trying to study?  

On Sun, Sep 28, 2014 at 2:42 PM, biomiky <michelestefanoli@gmail.com> wrote:

Hi folks!

Anybody knows how much costs in terms of consumables, capillary, tank, maintenance, solid phase... ecc a Gas Chromatography equipment? 

After buying a used one (found one for 1200 euros), who would be the stakeholders interested in the business? I would personally like to do some metabolomic research.

Thanks

Bye

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