Re: [DIYbio] Gas Chromatograph (DIY) maintenance

Thanks. Your tips are very appreciated. So I can have fun with fugal and plants estracts!

Il 28/set/2014 21:22 "Dakota Hamill" <dkotes@gmail.com> ha scritto:

Save yourself the money.  A GC is the last machine I'd recommend for steroid quantification.  A LC MS/MS would be the gold standard, and those are $50,000+ (for the old ones).  Not to mention consumables, gas tanks, regulators, solvent, and an actual lab space to do it in with dedicated high voltage lines.

Body fluids generally require processing and are time sensitive and temperature sensitive depending on what you are testing for.

Not to mention if you start handling anyone elses bodily fluids you'll need probably a handful of licenses and certifications.

Honestly it sounds like an idea you haven't really thought out in the least bit.

If I got an old GC i'd be playing with esters, ketones, and alcohols in studying perfumes or essential oil / flower extracts as they smell good (and it also means they are volatile and prime for a GC system).

Standards are also probably cheap enough that you could at least use retention time of known controls to compare to what you have in a plant extract.  

http://www.perkinelmer.com/CMSResources/Images/44-155954APP_011482_01_CharacterizationofPerfumeFragrances.pdf

I don't know where you live but some people are super into herbal extracts and things so I guess you could do GC runs of their sample mixtures and identify key chemical components.  I can't imagine you'd get rich off of it though.

On Sun, Sep 28, 2014 at 3:02 PM, Michele Stefanoli <michelestefanoli@gmail.com> wrote:

Not bought yet. Its only gc. I would study body fluids. Does the sample need long processing? Is possible to diagnose some conditions like detect hormones?

Il 28/set/2014 20:54 "Dakota Hamill" <dkotes@gmail.com> ha scritto:

It can vary.

Can you link the machine you bought?  Or, make model and manufacturer?

Did it come with software?  Is it even working?  Is it the really old kind that just uses a direct print-out?

You'll need a carrier gas and collision gas, so you have to be somewhere where they'll even drop off nitrogen, air, or helium tanks.  

Actually just read it's a GC not a GCMS, so forget collision gasses. We had a GC from the 50's or 60's that still worked.  Pretty simple.  Turn on heat and carrier gas, inject, old school perforated print out, integrate peaks by hand.  

I find LC's to be 100x more useful than GC's, unless you are working with small organic molecules that are volatile. 

Did you have a specific class of metabolites you are wanting to look at with GC?    Where are you going to find controls for your compounds? 

I guess the most important question is what are you even really trying to study?  

On Sun, Sep 28, 2014 at 2:42 PM, biomiky <michelestefanoli@gmail.com> wrote:

Hi folks!

Anybody knows how much costs in terms of consumables, capillary, tank, maintenance, solid phase... ecc a Gas Chromatography equipment? 

After buying a used one (found one for 1200 euros), who would be the stakeholders interested in the business? I would personally like to do some metabolomic research.

Thanks

Bye

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