Re: [DIYbio] Re: Fish gDNA isolation

The columns are just a convenient way to pass your sample over
diatomaceous earth. Get some diatomaceous earth powder and wash it
with your DNA binding buffer. Then add it you your sample and mix it,
then centrifuge to pellet the powder and pipette off the supernatant.
It's less fool proof since you can potentially suck up the sample, but
way cheaper.

You'll still need all of the same solutions but those you can often
make yourself for pretty cheap, if you can find the recipes. A lot of
the Qiagen recipes are on the interwebs.

I should mention, I've never tried this with genomic DNA. I always
use it for minipreps and it works just as well as the columns

-cory

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