Re: [DIYbio] Re: Karyotyping? etc

Check out colchicine and giemsa stain, the first sets up cultured
cells for karyotyping and the latter stains the chromosomes.

www.pitt.edu/~super7/31011-32001/31081.ppt

If you don't have the time:
https://www.scienceexchange.com/services/karyotyping



On Sep 23, 2014 7:03 AM, "jem" <jemmar...@gmail.com> wrote:
>
> Hi dakota,
>
> I've had a lot of weird medical problems, the details of which I shan't go into. Yes I had surgeries when I was too young to remember and surgery later when I did remember but discovered later that they were doing something else.. The surgeries probably explain the development issues I had and medical problems encountered later on. I've had multiple scans, nothing too strange encountered, just minor stuff related to development issues, its been suggested I have an mri but I expect nothing significant will be found and again its another cost I can't really afford and another test I don't really want (I'm pretty sick of hospitals, pun intended)
>
> For me this is more out of curiosity and eliminating a possibility.
>
> One endro suggested a probable scenario and he could be right but I kind of disagree, he was speculating, and I would like to see proof, so I gave bloods for analysis, a karyotype test was supposed to have been carried out but it was either 1. not done or 2. they lost the paperwork (which is quite normal here).
>
>
>
>
>
>
> On Tuesday, September 23, 2014 12:59:23 PM UTC+1, Dakota wrote:
>>
>> Yes it is unfortunately sometimes difficult to find a doctor who will listen to what you have to say with an open mind, and not just an open ear.  I've had the best luck at teaching hospitals, although that can differ I am sure.  I'd say showing up with knowledge in hand ready to propose a hypothesis and subsequent specific test might be your best bet in some cases.   I've had the best luck showing up with knowledge in hand or head and approaching the subject with delivery in medical or chemical terms, and in general have gotten a good response once the doctor realizes you aren't another Joe Smoe reading WebMD and convincing yourself you have cancer and 100 other diseases.
>>
>> Or, maybe they are silently still thinking they know better but will appease the patient to give the illusion of them being cooperative and believing anything you just said.
>>
>> I don't know medicine so I can't comment fully, but I would say that scientists would probably make pretty damn good doctors.  The lack of analysis and data sets gathered prior to a diagnosis and treatment regiment I've experienced is pretty scary.  At least at the teaching hospital, the specialist wanted 3 sets of tests spaced 1 week apart to get an average of levels, whereas another one took one set of tests and said here, take this forever.
>>
>> There is also the problem of waiting months in between seeing specialists, and if one sucks, you still have to pay!
>>
>> But anyway, for your specific case, just keep googling and finding tons of papers relating to what it is you want to do.  I think a lot of the legwork is going to have to be done by you, if you want something bad enough, you'll figure out a way to do it.  Obviously having a personal relationship to the cause is an even bigger motivator.
>>
>> I guess I'm still not entirely sure what exactly you want to come up with a test for, just gonadal dysgensis or other intersex conditions in general?
>>
>> When you say hide surgeries, do you mean you had procedures done when you were to young to remember and your doctors/parents didn't want you to know about them for fear they might impact your perception of yourself?
>>
>> Sorry if that's a personal question but I'm just trying to understand where you're coming from.
>>
>>
>>
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