Re: [DIYbio] Re: Karyotyping? etc

Hey.

Not to say that you should not do karyotyping if you'd really like to,
but it might be easier (and a lot more reliable) to rather look at the
DNA sequence. There are companies that do genotyping for just 100
dollars, and that gives you a lot of additional fun and medical
information. Or if you like DIY, and have access to a PCR machine,
doing a few PCRs with primers specific to Y chromosome should be very
easy, cheap and reliable.

On Thu, Sep 25, 2014 at 11:43 AM, jem <jemmaredmond@gmail.com> wrote:
> coolio
>
> Thanks for the info, it looks like one very convoluted process and perhaps
> time critical? I'd have to steal er borrow stuff from the other labs/groups
> plus I would need to book time on the microscopes.. It would be good to see
> this process being done in minute detail like a video or something. I'll
> have a gander and see if I can find some more info later...
>
> On Tuesday, September 23, 2014 7:49:23 PM UTC+1, Nathan McCorkle wrote:
>>
>> Check out colchicine and giemsa stain, the first sets up cultured
>> cells for karyotyping and the latter stains the chromosomes.
>>
>> www.pitt.edu/~super7/31011-32001/31081.ppt
>>
>> If you don't have the time:
>> https://www.scienceexchange.com/services/karyotyping
>>
>>
>>
>> On Sep 23, 2014 7:03 AM, "jem" <jemmar...@gmail.com> wrote:
>> >
>> > Hi dakota,
>> >
>> > I've had a lot of weird medical problems, the details of which I shan't
>> > go into. Yes I had surgeries when I was too young to remember and surgery
>> > later when I did remember but discovered later that they were doing
>> > something else.. The surgeries probably explain the development issues I had
>> > and medical problems encountered later on. I've had multiple scans, nothing
>> > too strange encountered, just minor stuff related to development issues, its
>> > been suggested I have an mri but I expect nothing significant will be found
>> > and again its another cost I can't really afford and another test I don't
>> > really want (I'm pretty sick of hospitals, pun intended)
>> >
>> > For me this is more out of curiosity and eliminating a possibility.
>> >
>> > One endro suggested a probable scenario and he could be right but I kind
>> > of disagree, he was speculating, and I would like to see proof, so I gave
>> > bloods for analysis, a karyotype test was supposed to have been carried out
>> > but it was either 1. not done or 2. they lost the paperwork (which is quite
>> > normal here).
>> >
>> >
>> >
>> >
>> >
>> >
>> > On Tuesday, September 23, 2014 12:59:23 PM UTC+1, Dakota wrote:
>> >>
>> >> Yes it is unfortunately sometimes difficult to find a doctor who will
>> >> listen to what you have to say with an open mind, and not just an open ear.
>> >> I've had the best luck at teaching hospitals, although that can differ I am
>> >> sure. I'd say showing up with knowledge in hand ready to propose a
>> >> hypothesis and subsequent specific test might be your best bet in some
>> >> cases. I've had the best luck showing up with knowledge in hand or head
>> >> and approaching the subject with delivery in medical or chemical terms, and
>> >> in general have gotten a good response once the doctor realizes you aren't
>> >> another Joe Smoe reading WebMD and convincing yourself you have cancer and
>> >> 100 other diseases.
>> >>
>> >> Or, maybe they are silently still thinking they know better but will
>> >> appease the patient to give the illusion of them being cooperative and
>> >> believing anything you just said.
>> >>
>> >> I don't know medicine so I can't comment fully, but I would say that
>> >> scientists would probably make pretty damn good doctors. The lack of
>> >> analysis and data sets gathered prior to a diagnosis and treatment regiment
>> >> I've experienced is pretty scary. At least at the teaching hospital, the
>> >> specialist wanted 3 sets of tests spaced 1 week apart to get an average of
>> >> levels, whereas another one took one set of tests and said here, take this
>> >> forever.
>> >>
>> >> There is also the problem of waiting months in between seeing
>> >> specialists, and if one sucks, you still have to pay!
>> >>
>> >> But anyway, for your specific case, just keep googling and finding tons
>> >> of papers relating to what it is you want to do. I think a lot of the
>> >> legwork is going to have to be done by you, if you want something bad
>> >> enough, you'll figure out a way to do it. Obviously having a personal
>> >> relationship to the cause is an even bigger motivator.
>> >>
>> >> I guess I'm still not entirely sure what exactly you want to come up
>> >> with a test for, just gonadal dysgensis or other intersex conditions in
>> >> general?
>> >>
>> >> When you say hide surgeries, do you mean you had procedures done when
>> >> you were to young to remember and your doctors/parents didn't want you to
>> >> know about them for fear they might impact your perception of yourself?
>> >>
>> >> Sorry if that's a personal question but I'm just trying to understand
>> >> where you're coming from.
>> >>
>> >>
>> >>
>> > --
>> > -- You received this message because you are subscribed to the Google
>> > Groups DIYbio group. To post to this group, send email to
>> > diy...@googlegroups.com. To unsubscribe from this group, send email to
>> > diybio+un...@googlegroups.com. For more options, visit this group at
>> > https://groups.google.com/d/forum/diybio?hl=en
>> > Learn more at www.diybio.org
>> > ---
>> > You received this message because you are subscribed to the Google
>> > Groups "DIYbio" group.
>> > To unsubscribe from this group and stop receiving emails from it, send
>> > an email to diybio+un...@googlegroups.com.
>> > To post to this group, send email to diy...@googlegroups.com.
>> > Visit this group at http://groups.google.com/group/diybio.
>> > To view this discussion on the web visit
>> > https://groups.google.com/d/msgid/diybio/b15b4d50-7954-49ea-85cc-21e5446481a7%40googlegroups.com.
>> > For more options, visit https://groups.google.com/d/optout.
>
> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+unsubscribe@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio.
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/1717e021-0fa0-41b6-857a-ec4ed01e6174%40googlegroups.com.
>
> For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAB9v_DFUT0voaJ109bO%2B8%2B4JXWyeJB3quARg5B_WpraGzSOGnA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.