Hey Stacy,
If I were to order the plant today it would take a few weeks to arrive.
In interim, how could I go about prepping? I'm a novice and am not extensively familiar with these terms and procedures. How would I go about making a large insert shotgun library and the following steps of plasmid preps and end-sequencing? not to mention, what exactly would the end sequencing help me accomplish?
Its easy to order the plants online, seeds as well. however I'm not sure when I will have a blooming flower (tissue), once I receive seeds/plant. Its cultivated.
Thanks,
Nico
On Tuesday, November 25, 2014 12:06:44 PM UTC-8, SC wrote:
-- If I were to order the plant today it would take a few weeks to arrive.
In interim, how could I go about prepping? I'm a novice and am not extensively familiar with these terms and procedures. How would I go about making a large insert shotgun library and the following steps of plasmid preps and end-sequencing? not to mention, what exactly would the end sequencing help me accomplish?
Its easy to order the plants online, seeds as well. however I'm not sure when I will have a blooming flower (tissue), once I receive seeds/plant. Its cultivated.
Thanks,
Nico
On Tuesday, November 25, 2014 12:06:44 PM UTC-8, SC wrote:
Hi Nico,The cheapest way to get going would be to make a large insert shotgun library. You'll need access to live plant tissue for that; if you don't have access to live plant tissue, you can make a small insert library. That would be followed by some plasmid preps (not expensive), and some end-sequencing (a little expensive).I'd be happy to help you through the project.To start with, do you have access to live tissue? Is this species wild-collected or cultivated?Stacy
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