[DIYbio] Re: Need some help starting a project (Agrobacteria-mediated transformation of a dicot via floral dip)

Ah I forgot to mention, you can also try a transient transformation. Just streak the leaves with the agobacterium solution, and they will get the gene inserted. However, the leaves get brown after x weeks, and die off. So the whole plant is not a transgene, it's a mosaic. Still nice to try with GFP. For some plants you cann add vanillin to agrobacterium to enhance the aggressivity (it acts as a wound signal). 

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