If you focus on one project, it may take you not that long for a first proof of concept?
-- If you have chemical knowledge already, say you want to produce ethanol from glucose.
First you identify an efficient pathway from nature (out of the literature).
Glucose ----(Enzyme1)----> Pyruvate ----(Enzyme2)-----> Ethanal -----(Enzyme3) ----> Ethanol
Enzyme 1 already is in the organism. So you have to express Enzyme 2 and Enzyme 3 to make the organism produce ethanol.
Easiest way: Have these genes synthesized in a codon usage that your organism efficiently translates and insert the DNA into that organism.
Attention, if you are outside USA that may be illegal.
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