Re: [DIYbio] Cheapest sequencing deals?

Just under 5.

Sequencing companies are usually willing to negotiate. If you can
guarantee enough volume or submit 96 well plates instead of single
tubes or agree to only pay with check/PO (save them the credit card
fees) then you can usually get a discount. Although, 3 USD is pretty
cheap already. I'm not sure you'll be able to shave off any more.

One trick I've used before: if you expect 9 out of 10 clones to be
good and you're just trying to weed out the bad 10% you can pool
samples together. In the first 400 or 500 (where the signal is really
clean) it will be obvious in the trace where the pooled samples
disagree. Instead of getting a single large peak on one channel you'll
see two smaller peaks on two channels. Toss out the bad pools and
keep the samples from the good pools. This won't work if you're
expecting a lot of variation between clones.

-cory

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