Hi,
-- I am doing a science project on how various compounds affect C. elegans inflicted with Parkinson's.
I am using strain OW13 of C. elegans, in which aSynuclein (an indicator of Parkinson's) glows yellow.
The compounds which I hope to test are in powdered forms. They are punicaligan, curcumin, and nicotinamide (all from Sigma).
Also, the strains arrive feeding on an E. coli lawn set in NGM (Nematode Growth Medium) Agar.
I am not sure how to insert the compounds into the diet of the worms.
I currently have three ideas:
1) Adding the powder directly onto the C. elegens/E. coli lawn.
2) Adding the powder to a culture of E. coli and feeding the C. elegans this diet.
3) Adding the powder directly onto the NGM agar.
Which method you think is best, or is there a better way to add these compounds to the C. elegans' diet?
Thanks for the help!
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