Thank you all for quick reply!
So i decided to make two plates, one with PDA and one with Marmite just to check if it works!
I have applied as much safety precautions as I could but these are my first plates so probably not enough safety :) , i will make few more since i've got plenty of agar left. I have sterelized the plates in an oven, i've got autoclave tape so i'm sure it have reached the temperature. used the alcohol burner and 95% alcohol to sterelize the spreader and needle(syrienge with the fungus) I also have hepa filtered air purifier on the desk aimed at the plates.
I have placed them into my DIY incubator. cost me £10 to make it. I lined styrofoam box with aluminium foil and placed reptile heating pad(cheapest one with temperature control on ebay £1.59!) and for keeping eye on temperature I have placed cheap digital thermometer! I was able to keep the temperature of 35 degrees celcius for few days without any trouble
-- So i decided to make two plates, one with PDA and one with Marmite just to check if it works!
I have applied as much safety precautions as I could but these are my first plates so probably not enough safety :) , i will make few more since i've got plenty of agar left. I have sterelized the plates in an oven, i've got autoclave tape so i'm sure it have reached the temperature. used the alcohol burner and 95% alcohol to sterelize the spreader and needle(syrienge with the fungus) I also have hepa filtered air purifier on the desk aimed at the plates.
I have placed them into my DIY incubator. cost me £10 to make it. I lined styrofoam box with aluminium foil and placed reptile heating pad(cheapest one with temperature control on ebay £1.59!) and for keeping eye on temperature I have placed cheap digital thermometer! I was able to keep the temperature of 35 degrees celcius for few days without any trouble
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