Re: [DIYbio] Best/Cheapest Services for DNA Sequencing & Synthesis

Hi all,

So where does an independent DIYbio lab find the best deals when it comes to DNA sequencing and synthesis? Market places like Science Exchange offer tons of services that we could avail ourselves from, but figuring out which is the best deal is often less than trivial. So let's put together some of our own favorites here...

We may want to skip the small stuff - ordering single primers, or getting some Sanger sequencing on a PCR product has become so commoditized that you can just about find them on any street corner. So let's look at some of the more cutting-edge applications:

• Who offers the cheapest / most convenient bacterial genome sequencing?

• Who offers the most affordable RNAseq services?

• Who offers the cheapest / most convenient 16S, ITS or other DNA barcoding services?
  

• What is the cheapest human genome sequencing available to DIYbio?
  

• Who has the best rate for buying relatively large numbers of 60nt oligos (e.g. for DNA origami)?
  

• What is the best rate we can find for longer oligos (>100nt, e.g. to encode text messages)?

• What is the best rate for gene synthesis, and longer DNA?
  

Please post your favorites!

Patrik

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